Chemistry of Living Systems Semiannual Report, May 1 - Sep. 30, 1966

Biochemical mechanisms of heredity and gene expression, their adaptation to environmental extremes, and possible relationships to origin and development of lif

Chemistry of living systems Semiannual report, Apr. 1 - Sep. 30, 1967

Biochemical studies on nucleic acids, proteins, metabolisms, bacteriophages, and related topic

Present Status of the Amino Acid Code

Protein composition studies and present status of amino acid cod

Repetitions in the polypeptide sequence of cytochromes

Protein evolution from peptides, gene duplications and deletions in polypeptides and cytochrome

Specific Nature of Hydrolysis of Insulin and Tobacco Mosaic Virus Protein by Thermolysin

Oxidized bovine insulin and tobacco mosaic virus protein used to determine hydrolysis specificity of thermolysi

Molecules and Evolution

Biochemical basis of evolution - evolution and DNA, genetic code, microbiology and heredity, mutations, and evolution and hemoglobin

Chemistry of living systems Semiannual report, 1 Oct. 1966 - 31 Mar. 1967

Reconstitution of ribosomes, evolution of enzyme systems in pseudomonas, molecular mechanism for T4 bacteriophage, and other studies related to evolution and chemistry of living system

Soft topographic map for clustering and classification of bacteria

In this work a new method for clustering and building a topographic representation of a bacteria taxonomy is presented. The method is based on the analysis of stable parts of the genome, the so-called “housekeeping genes”. The proposed method generates topographic maps of the bacteria taxonomy, where relations among different type strains can be visually inspected and verified. Two well known DNA alignement algorithms are applied to the genomic sequences. Topographic maps are optimized to represent the similarity among the sequences according to their evolutionary distances. The experimental analysis is carried out on 147 type strains of the Gammaprotebacteria class by means of the 16S rRNA housekeeping gene. Complete sequences of the gene have been retrieved from the NCBI public database. In the experimental tests the maps show clusters of homologous type strains and present some singular cases potentially due to incorrect classification or erroneous annotations in the database